Composite

Part:BBa_K2717010

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-10-09)


gdh-gfp-reverse

In this composite part, we link GFP and glucose dehydrogenase in reverse and use Promoter J23100 and RBS 2000 control the GFP and Promoter lacI control the glucose dehydrogenase and tag. Since GFP and gdh are arranged in reverse, the two genes express independently and don’t affect each other.

Since GFP and gdh are arranged in reverse and there are two terminators between them, the two genes express independently and don’t affect each other. We designed this part as a comparison to confirm that the introduction of TGATG allows the plasmid to highly express the gene of interest (the upstream gene of TGATG).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1157
    Illegal AgeI site found at 2049
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644
    Illegal BsaI.rc site found at 1175


[edit]
Categories
Parameters
None